myhc type 2a (Developmental Studies Hybridoma Bank)
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Developmental Studies Hybridoma Bank
myhc type 2a
Myhc Type 2a, supplied by Developmental Studies Hybridoma Bank, used in various techniques. Bioz Stars score: 96/100, based on 1084 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/myhc+2a/anti-Myosin+heavy+chain+Type+IIA/pm41276031-134-31-38
Average 96 stars, based on 1084 article reviews
Myhc Type 2a, supplied by Developmental Studies Hybridoma Bank, used in various techniques. Bioz Stars score: 96/100, based on 1084 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/myhc+2a/anti-Myosin+heavy+chain+Type+IIA/pm41276031-134-31-38
Average 96 stars, based on 1084 article reviews
myhc type 2a - by Bioz Stars,
2026-10
96/100 stars
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Incubation:Article Title: Polyamine metabolism dysregulation contributes to muscle fiber vulnerability in ALS Article Snippet: For immunofluorescence staining, 10mmmuscle cryosections were permeabilized with 100%acetone for 1min at room temperature, followed by blocking for 1 h with a blocking solution containing 4% BSA (MilliporeSigma, A7030-100G) in PBS 1X. .. Incubation with secondary antibody was carried out at room temperature for 1 h. The primary antibodies used for immunofluorescence included: anti-synaptophysin (1:300, Invitrogen, PA1-1043), anti-caveolin-3 (1:1000, BD Transduction Laboratories, 610420), antilaminin (1:300, MilliporeSigma, L9393) and mouse monoclonal antibodies from the Development Studies Hybridoma Bank (DSHB, Iowa, USA) for Article Title: CKD-Induced Oxidative Twitch Muscle Atrophy Is Mediated by TGF- β Article Snippet: .. Proteins were electrotransferred onto polyvinylidene fluoride membranes, blocked with 5% skim milk, and incubated with primary antibodies against PGC-1 α (Sigma, # ST1202-1SETCN), SMAD2/3 (Cell Signaling Technology, #8828), p-SMAD2/3 (Cell Signaling Technology, #8685), MyHC 1 (Developmental Studies Hybridoma Bank [DSHB], BA-F8), Article Title: CKD-Induced Oxidative Twitch Muscle Atrophy Is Mediated by TGF- β Article Snippet: .. Subsequently, sections were incubated with primary antibodies against laminin (sigma, cat#: L9393), MyHC 1 (DSHB, BA-F8), Immunofluorescence:Article Title: Polyamine metabolism dysregulation contributes to muscle fiber vulnerability in ALS Article Snippet: For immunofluorescence staining, 10mmmuscle cryosections were permeabilized with 100%acetone for 1min at room temperature, followed by blocking for 1 h with a blocking solution containing 4% BSA (MilliporeSigma, A7030-100G) in PBS 1X. .. Incubation with secondary antibody was carried out at room temperature for 1 h. The primary antibodies used for immunofluorescence included: anti-synaptophysin (1:300, Invitrogen, PA1-1043), anti-caveolin-3 (1:1000, BD Transduction Laboratories, 610420), antilaminin (1:300, MilliporeSigma, L9393) and mouse monoclonal antibodies from the Development Studies Hybridoma Bank (DSHB, Iowa, USA) for Bioprocessing:Article Title: Polyamine metabolism dysregulation contributes to muscle fiber vulnerability in ALS Article Snippet: For immunofluorescence staining, 10mmmuscle cryosections were permeabilized with 100%acetone for 1min at room temperature, followed by blocking for 1 h with a blocking solution containing 4% BSA (MilliporeSigma, A7030-100G) in PBS 1X. .. Incubation with secondary antibody was carried out at room temperature for 1 h. The primary antibodies used for immunofluorescence included: anti-synaptophysin (1:300, Invitrogen, PA1-1043), anti-caveolin-3 (1:1000, BD Transduction Laboratories, 610420), antilaminin (1:300, MilliporeSigma, L9393) and mouse monoclonal antibodies from the Development Studies Hybridoma Bank (DSHB, Iowa, USA) for other:Article Title: Adaptable Immunofluorescence Protocol for Muscle Fiber Typing in FFPE Human and Mouse Skeletal Muscle and Intact Mouse Hindlimbs Article Snippet: Monoclonal: Clone SC‐71 , Mouse isotype IgG1 , MyHC 2a , 5.58 μg/ml , Article Title: Polyamine metabolism dysregulation contributes to muscle fiber vulnerability in ALS Article Snippet: After immunostaining, sections were incubated with DAPI (Thermo Fisher Scientific, D1306) in PBS for 5 min for nuclear staining, washed in PBS, and mounted with glycerol (3:1 in PBS). Muscles:Article Title: Accumulation of lipids after acute direct and indirect traumatic injuries in male and female mice Article Snippet: Each frame was then stitched together using Nikon NIS-Elements AR software and analysis was completed on the full stitched image. .. Within the middle muscle section only, muscles were stained for myosin heavy chain (MyHC) isoform expression MyHC slow (DSHD Cat# BA-D5, RRID: AB_2235587; 5 μg/ml) and Staining:Article Title: Accumulation of lipids after acute direct and indirect traumatic injuries in male and female mice Article Snippet: Each frame was then stitched together using Nikon NIS-Elements AR software and analysis was completed on the full stitched image. .. Within the middle muscle section only, muscles were stained for myosin heavy chain (MyHC) isoform expression MyHC slow (DSHD Cat# BA-D5, RRID: AB_2235587; 5 μg/ml) and Expressing:Article Title: Accumulation of lipids after acute direct and indirect traumatic injuries in male and female mice Article Snippet: Each frame was then stitched together using Nikon NIS-Elements AR software and analysis was completed on the full stitched image. .. Within the middle muscle section only, muscles were stained for myosin heavy chain (MyHC) isoform expression MyHC slow (DSHD Cat# BA-D5, RRID: AB_2235587; 5 μg/ml) and Pyrolysis Gas Chromatography:Article Title: CKD-Induced Oxidative Twitch Muscle Atrophy Is Mediated by TGF- β Article Snippet: .. Proteins were electrotransferred onto polyvinylidene fluoride membranes, blocked with 5% skim milk, and incubated with primary antibodies against PGC-1 α (Sigma, # ST1202-1SETCN), SMAD2/3 (Cell Signaling Technology, #8828), p-SMAD2/3 (Cell Signaling Technology, #8685), MyHC 1 (Developmental Studies Hybridoma Bank [DSHB], BA-F8), Labeling:Article Title: CKD-Induced Oxidative Twitch Muscle Atrophy Is Mediated by TGF- β Article Snippet: .. Subsequently, sections were incubated with primary antibodies against laminin (sigma, cat#: L9393), MyHC 1 (DSHB, BA-F8), |
